Amphiphysin antibody (Internal Region)

Catalog No. ABIN185628

This anti-Amphiphysin antibody is a Goat Polyclonal antibody detecting Amphiphysin in WB, ELISA and IHC. Suitable for Human and Mouse.

Quick Overview for Amphiphysin antibody (Internal Region) (ABIN185628)

Target: Amphiphysin (AMPH)

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Amphiphysin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-Amphiphysin Antibody

Binding Specificity: Internal Region

Purpose: Amphiphysin / AMPH

Sequence: KRKDESRISK AE

Specificity: This antibody is expected to recognise both reported isoforms (NP_001626.1 and NP_647477.1).

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KRKDESRISKAE, from the internal region of the protein sequence according to NP_001626.1, NP_647477.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Cerebellum shows nuclear and fibrous staning. Recommended concentration, 2-4 μg/mL.

Western Blot: Approx 125 kDa band observed in mouse brain lysates (calculated MW of 76.3 kDa according to NP_001626.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Wigge et al, Mol Biol Cell. 1997

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Amphiphysin

Target: Amphiphysin (AMPH)

Alternative Name: AMPH

Background: AMPH, amphiphysin, amphiphysin (Stiff-Man syndrome with breast cancer 128 kDa autoantigen) , HGNC:471, AMPH1

Gene ID: 273, 218038

NCBI Accession: NP_001626, NP_647477