PREP antibody (Internal Region)

Catalog No. ABIN185633

The Goat Polyclonal anti-PREP antibody has been validated for ELISA and IHC. It is suitable to detect PREP in samples from Human.

Quick Overview for PREP antibody (Internal Region) (ABIN185633)

Target: PREP (Prolyl Endopeptidase (PREP))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PREP antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-PREP Antibody

Binding Specificity: Internal Region

Purpose: Prolyl Endopeptidase / PREP

Sequence: QYIVGRSRKQ SNP

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QYIVGRSRKQSNP, from the internal region of the protein sequence according to NP_002717.3.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney and Tonsil. Recommended concentration: 3.75 μg/mL.

Western Blot: Preliminary experiments gave an approx 45 kDa band in Human Brain, Lymph Node, Spleen and Testis lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe giv

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PREP

Target: PREP (Prolyl Endopeptidase (PREP))

Alternative Name: PREP

Background: PREP, prolyl endopeptidase , HGNC:9358, MGC16060, PE, PEP , OTTHUMP00000040498, dJ355L5.1 (prolyl endopeptidase), post-proline cleaving enzyme

Gene ID: 5550, 19072, 83471

NCBI Accession: NP_002717