WNT3 antibody (Internal Region)

Catalog No. ABIN185634

This anti-WNT3 antibody is a Goat Polyclonal antibody detecting WNT3 in WB, ELISA and IHC. Suitable for Human. This Primary Antibody has been cited in 2+ publications.

Quick Overview for WNT3 antibody (Internal Region) (ABIN185634)

Target: WNT3 (Wingless-Type MMTV Integration Site Family, Member 3 (WNT3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This WNT3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-WNT3 Antibody

Binding Specificity: Internal Region

Purpose: WNT3

Sequence: CGRGHNTRTE KRKEK

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CGRGHNTRTEKRKEK, from the internal region of the protein sequence according to NP_110380.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 40 kDa band observed in lysates of cell line HEK293 (calculated MW of 39.6 kDa according to NP_110380.1). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-WNT3 antibody (ABIN185634)

Fujimaki, Hidaka, Asashima, Takemasa, Kuwabara: "Wnt protein-mediated satellite cell conversion in adult and aged mice following voluntary wheel running." in: The Journal of biological chemistry, Vol. 289, Issue 11, pp. 7399-412, (2014) (PubMed).

Okamoto, Inoue, Iwamura, Terashima, Soya, Asashima, Kuwabara: "Reduction in paracrine Wnt3 factors during aging causes impaired adult neurogenesis." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 10, pp. 3570-82, (2011) (PubMed).

Target Details for WNT3

Target: WNT3 (Wingless-Type MMTV Integration Site Family, Member 3 (WNT3))

Alternative Name: WNT3

Background: WNT3 , wingless-type MMTV integration site family, member 3 , INT4, MGC131950, MGC138321, MGC138323 , WNT-3 proto-oncogene protein precursor

Gene ID: 7473, 22415, 24882

NCBI Accession: NP_110380

Pathways: WNT Signaling, Regulation of Cell Size