Ezrin antibody (Internal Region)

Catalog No. ABIN185664

This anti-Ezrin antibody is a Goat Polyclonal antibody detecting Ezrin in WB, ELISA and IHC. Suitable for Human.

Quick Overview for Ezrin antibody (Internal Region) (ABIN185664)

Target: Ezrin (EZR)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Ezrin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-Ezrin Antibody

Binding Specificity: Internal Region

Purpose: Ezrin / villin 2

Sequence: QDYEEKTKKA ER

Cross-Reactivity: Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QDYEEKTKKAER, from the internal region of the protein sequence according to NP_003370.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Colon and Kidney. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 80 kDa band observed in Human Placenta lysates (calculated MW of 69.4 kDa according to NP_003370.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zhu et al, Am J Physiol Cell Ph

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Ezrin

Target: Ezrin (EZR)

Alternative Name: EZR

Background: Villin 2 (ezrin) , ezrin, VIL2, CVIL, CVL, DKFZp762H157, FLJ26216, MGC1584 , cytovillin, villin 2

Gene ID: 7430, 22350, 54319

NCBI Accession: NP_003370

Pathways: Maintenance of Protein Location