GPX4 antibody (C-Term)

Catalog No. ABIN185669

The Goat Polyclonal anti-GPX4 antibody has been validated for WB, IHC, ELISA and IP. It is suitable to detect GPX4 in samples from Human. There are 2+ publications available.

Quick Overview for GPX4 antibody (C-Term) (ABIN185669)

Target: GPX4 (Glutathione Peroxidase 4 (GPX4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GPX4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Grade: Verified

Product Details anti-GPX4 Antibody

Binding Specificity: C-Term

Purpose: GPX4 (Isoform a and c)

Sequence: EEPLVIEKDL PHY

Specificity: This antibody is expected to recognise isoform A (NP_002076.2) and isoform C (NP_001034937.1).

Cross-Reactivity: Cow, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EEPLVIEKDLPHY, from the C Terminus of the protein sequence according to NP_002076.2, NP_001034937.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Testis shows strong staining surrounding the nuclei of spermatocytes and surrounding spermatozoa. Recommended concentration: 0.3 μg/mL.

Western Blot: Approx 19 kDa band observed in Human Kidney and Testis lysates and in lysates of cell line HEK293 (calculated MW of 22.1 kDa according to NP_002076.2). Recommended concentration: 0.1-0.3 μg/mL. This antibody has been successfully used in WB on

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunoprecipitation:This antibody has been successfully used in IP on Human: Schoenmakers E et al. (2010) PMID:21084748.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-GPX4 antibody (ABIN185669)

Papp, Lu, Bolderson, Boucher, Singh, Holmgren, Khanna: "SECIS-binding protein 2 promotes cell survival by protecting against oxidative stress." in: Antioxidants & redox signaling, Vol. 12, Issue 7, pp. 797-808, (2010) (PubMed).

Schoenmakers, Agostini, Mitchell, Schoenmakers, Papp, Rajanayagam, Padidela, Ceron-Gutierrez, Doffinger, Prevosto, Luan, Montano, Lu, Castanet, Clemons, Groeneveld, Castets, Karbaschi, Aitken, Dixon et al.: "Mutations in the selenocysteine insertion sequence-binding protein 2 gene lead to a multisystem selenoprotein deficiency disorder in humans. ..." in: The Journal of clinical investigation, Vol. 120, Issue 12, pp. 4220-35, (2010) (PubMed).

Target Details for GPX4

Target: GPX4 (Glutathione Peroxidase 4 (GPX4))

Alternative Name: GPX4

Background: GPX4, glutathione peroxidase 4 (phospholipid hydroperoxidase) , HGNC:4556, PHGPx, snGPx, glutathione peroxidase 4, phospholipid hydroperoxidase, sperm nucleus glutathione peroxidase

Gene ID: 2879

NCBI Accession: NP_002076, NP_001034937