WT1 antibody (N-Term)

Catalog No. ABIN185674

This Goat Polyclonal antibody specifically detects WT1 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for WT1 antibody (N-Term) (ABIN185674)

Target: WT1 (Wilms Tumor 1 (WT1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This WT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-WT1 Antibody

Binding Specificity: N-Term

Purpose: Wilms tumor 1 / WT1

Sequence: QDPASTCVPE PASQH

Specificity: This antibody is expected to recognise all four reported isoforms (NP_000369.3, NP_077742.2, NP_077743.2, NP_077744.3).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence QDPASTCVPEPASQH, from the N Terminus of the protein sequence according to NP_000369.3, NP_077742.2, NP_077743.2, NP_077744.3.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 55 kDa band observed in Human Kidney, Spleen and Testis lysates (calculated MW of 54.3 kDa according to NP_000369.3). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Alternative Name: WT1

Background: WT1, Wilms tumor 1, GUD, WAGR, WIT-2, WT33

Gene ID: 7490

NCBI Accession: NP_000369, NP_077742, NP_077743, NP_077744

Pathways: Tube Formation