SEMA5A antibody (Internal Region)

Catalog No. ABIN185680

This Goat Polyclonal antibody specifically detects SEMA5A in ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for SEMA5A antibody (Internal Region) (ABIN185680)

Target: SEMA5A (Sema Domain, Seven thrombospondin Repeats (Type 1 and Type 1-Like), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 5A (SEMA5A))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SEMA5A antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-SEMA5A Antibody

Binding Specificity: Internal Region

Purpose: Semaphorin 5A

Sequence: DEATKKACYS KGKS

Specificity: The immunizing peptide corresponds to a part of the extracellular domain.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence DEATKKACYSKGKS, from the internal region of the protein sequence according to NP_003957.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Kidney shows membanous and vesicular staining in PCT cells. Recommended concentration: 4-6 μg/mL.

Western Blot: Preliminary experiments gave an approx 90 kDa in Mouse Brain lysates and approx 25 kDa band in Human Brain (Cerebellum), Colon, Heart and Kidney lysates after 0.03 μg/mL antibody staining. Please note that currently we cannot find an explanati

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SEMA5A

Target: SEMA5A (Sema Domain, Seven thrombospondin Repeats (Type 1 and Type 1-Like), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 5A (SEMA5A))

Alternative Name: SEMA5A

Background: SEMA5A, sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A, HGNC:10736, SEMAF, semF, sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane

Gene ID: 9037, 20356, 310207

NCBI Accession: NP_003957