FXR1 antibody (Internal Region)

Catalog No. ABIN185683

This Goat Polyclonal antibody specifically detects FXR1 in WB, ELISA, IF and FACS. It exhibits reactivity toward Human and Mouse.

Quick Overview for FXR1 antibody (Internal Region) (ABIN185683)

Target: FXR1 (Fragile X Mental Retardation, Autosomal Homolog 1 (FXR1))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This FXR1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-FXR1 Antibody

Binding Specificity: Internal Region

Purpose: FXR1

Sequence: RIEGDNENKL PRED

Specificity: This antibody is expected to recognise all reported isoforms (NP_005078.2, NP_001013456.1 and NP_001013457.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RIEGDNENKLPRED, from the internal region of the protein sequence according to NP_005078.2, NP_001013456.1, NP_001013457.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 75+80 kDa band observed in lysates of cell line NIH-3T3 (calculated MW of 59.9 kDa according to NP_001013457.1). The observed molecular weight corresponds to earlier findings in literature (Khandjian et al Hum Mol Genet. 1998 Dec,7(13

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration:

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for FXR1

Target: FXR1 (Fragile X Mental Retardation, Autosomal Homolog 1 (FXR1))

Alternative Name: FXR1

Background: FXR1, fragile X mental retardation, autosomal homolog 1 , fragile X mental retardation-related protein 1

Gene ID: 8087, 14359, 361927

NCBI Accession: NP_005078, NP_001013456, NP_001013457