VPS29 antibody (Internal Region)

Catalog No. ABIN185686

This Goat Polyclonal antibody specifically detects VPS29 in ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for VPS29 antibody (Internal Region) (ABIN185686)

Target: VPS29 (Vacuolar Protein Sorting 29 (VPS29))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This VPS29 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-VPS29 Antibody

Binding Specificity: Internal Region

Purpose: VPS29 (Internal)

Sequence: CTGNLCTKES YDYLK

Specificity: This antibody is expected to recognise both reported isoforms (NP_057310.1 and NP_476528.1).

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CTGNLCTKESYDYLK, from the internal region of the protein sequence according to NP_057310.1, NP_476528.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Uterus and Placenta. Recommended concentration: 3.75 μg/mL.

Western Blot: Preliminary experiments gave an approx 60 kDa band in Human Spleen lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 20.5

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for VPS29

Target: VPS29 (Vacuolar Protein Sorting 29 (VPS29))

Alternative Name: VPS29

Background: VPS29, vacuolar protein sorting 29 (yeast) , DC15, DC7, DKFZp564F0223, FLJ20492, PEP11 , retromer protein, vacuolar protein sorting 29, vacuolar protein sorting 29 (yeast homolog), vacuolar sorting protein VPS29/PEP11, x 007 protein

Gene ID: 51699, 56433, 288666

NCBI Accession: NP_057310, NP_476528