VPS29 antibody (Internal Region)

Catalog No. ABIN185686

Product Details anti-VPS29 Antibody

Target: VPS29 (Vacuolar Protein Sorting 29 (VPS29))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This VPS29 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Purpose: VPS29 (Internal)

Sequence: CTGNLCTKES YDYLK

Specificity: This antibody is expected to recognise both reported isoforms (NP_057310.1 and NP_476528.1).

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence CTGNLCTKESYDYLK, from the internal region of the protein sequence according to NP_057310.1, NP_476528.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Uterus and Placenta. Recommended concentration: 3.75 μg/mL.

Western Blot: Preliminary experiments gave an approx 60 kDa band in Human Spleen lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 20.5

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for VPS29

Target: VPS29 (Vacuolar Protein Sorting 29 (VPS29))

Alternative Name: VPS29 (VPS29 Products)

Background: VPS29, vacuolar protein sorting 29 (yeast) , DC15, DC7, DKFZp564F0223, FLJ20492, PEP11 , retromer protein, vacuolar protein sorting 29, vacuolar protein sorting 29 (yeast homolog), vacuolar sorting protein VPS29/PEP11, x 007 protein

Gene ID: 51699, 56433, 288666

NCBI Accession: NP_057310, NP_476528