FE65 antibody (C-Term)

Catalog No. ABIN185689

This Goat Polyclonal antibody specifically detects FE65 in WB and ELISA. It exhibits reactivity toward Mouse.

Quick Overview for FE65 antibody (C-Term) (ABIN185689)

Target: FE65 (APBB1) (Amyloid beta (A4) Precursor Protein-Binding, Family B, Member 1 (Fe65) (APBB1))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This FE65 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-FE65 Antibody

Binding Specificity: C-Term

Purpose: APBB1 / FE65

Sequence: GSLKPKRLGA HTP

Specificity: This antibody is expected to recognise both reported isoforms (NP_001155.1 and NP_663722.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-GSLKPKRLGAHTP, from the C Terminus of the protein sequence according to NP_001155.1, NP_663722.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 100 kDa band observed in lysates of NIH/3T3 (calculated MW of 77.2 kDa according to NP_001155.1 and 77.0 kDa according to NP_663722.1). The observed molecular weight corresponds to earlier findings in literature with different antibodi

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for FE65

Target: FE65 (APBB1) (Amyloid beta (A4) Precursor Protein-Binding, Family B, Member 1 (Fe65) (APBB1))

Alternative Name: APBB1

Background: APBB1, FE65, amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65), MGC:9072, RIR , adaptor protein FE65a2, amyloid beta A4 precursor protein-binding, family B, member 1, stat-like protein

Gene ID: 322

NCBI Accession: NP_001155, NP_663722

Pathways: Positive Regulation of Response to DNA Damage Stimulus