NOD2 antibody (Internal Region)

Catalog No. ABIN185703

This anti-NOD2 antibody is a Goat Polyclonal antibody detecting NOD2 in WB and ELISA. Suitable for Human.

Quick Overview for NOD2 antibody (Internal Region) (ABIN185703)

Target: NOD2 (Nucleotide-Binding Oligomerization Domain Containing 2 (NOD2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NOD2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-NOD2 Antibody

Binding Specificity: Internal Region

Purpose: CARD15 / NOD2 (Internal)

Sequence: KFRFTDRERH CSPTD

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence KFRFTDRERHCSPTD, from the internal region of the protein sequence according to NP_071445.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 100 kDa band observed in lysates of Human Peripheral Blood Mononucleocytes (calculated MW of 115 kDa according to NP_071445.1). Recommended concentration: 0.1-0.3 μg/mL. An additional band of unknown identity was also consistently obse

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NOD2

Target: NOD2 (Nucleotide-Binding Oligomerization Domain Containing 2 (NOD2))

Alternative Name: NOD2

Background: CARD15, NOD2, caspase recruitment domain family, member 15, ACUG, BLAU, CD, IBD1, NOD2B, PSORAS1, LRR-containing protein, NOD2 protein, caspase recruitment domain protein 15, inflammatory bowel disease protein 1, nucleotide-binding oligomerization domain

Gene ID: 64127, 257632, 291912

NCBI Accession: NP_071445

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome