SECISBP2 antibody (Internal Region)

Catalog No. ABIN185714

The Goat Polyclonal anti-SECISBP2 antibody has been validated for WB, ELISA, IF, IHC, IP and ICC. It is suitable to detect SECISBP2 in samples from Human and Mouse. There are 2+ publications available.

Quick Overview for SECISBP2 antibody (Internal Region) (ABIN185714)

Target: SECISBP2 (SECIS Binding Protein 2 (SECISBP2))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This SECISBP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Grade: Verified

Product Details anti-SECISBP2 Antibody

Binding Specificity: Internal Region

Purpose: SBP2

Sequence: KERQERKQRL QEN

Cross-Reactivity: Cow, Dog, Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-KERQERKQRLQEN

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Testis shows granular cytoplasm staining in Leydig cells. Recommended concentration, 3 μg/mL.

Western Blot: Approx 120 kDa band detected in HFF cell lysates. Data kindly provided by Laura Papp and Kum Kum Khanna. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Papp et al, Mol Cell Biol. 2006 J

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunocytochemistry/Immunofluorescence: This antibody has been successfully used in ICC/IF for C33A cells as described in the following paper: Papp et al, Nucleic Acids Res. 2008 Dec,36(22):7192-206, PMID: 19004874.
Immunoprec

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-SECISBP2 antibody (ABIN185714)

Raman, Pitts, Seyedali, Hashimoto, Bellinger, Berry: "Selenoprotein W expression and regulation in mouse brain and neurons." in: Brain and behavior, Vol. 3, Issue 5, pp. 562-74, (2014) (PubMed).

Papp, Wang, Kennedy, Boucher, Zhang, Gladyshev, Singh, Khanna: "Functional characterization of alternatively spliced human SECISBP2 transcript variants." in: Nucleic acids research, Vol. 36, Issue 22, pp. 7192-206, (2008) (PubMed).

Target Details for SECISBP2

Target: SECISBP2 (SECIS Binding Protein 2 (SECISBP2))

Alternative Name: SECISBP2

Background: SBP2 , RP11-89K14.1, SECISBP2, SECIS binding protein 2 , DKFZp686C09169, OTTHUMP00000064929, OTTHUMP00000064930, OTTHUMP00000064931, OTTHUMP00000064932, selenocysteine insertion sequence binding protein 2

Gene ID: 79048

NCBI Accession: NP_076982