PICK1 antibody (Internal Region)

Catalog No. ABIN185725

This Goat Polyclonal antibody specifically detects PICK1 in WB, IHC and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for PICK1 antibody (Internal Region) (ABIN185725)

Target: PICK1 (Protein Interacting With Protein Kinase C, alpha 1 (PICK1))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This PICK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-PICK1 Antibody

Binding Specificity: Internal Region

Purpose: PICK1

Sequence: HYNKLQADPK Q

Specificity: Reported variants represent identical protein (NP_036539.1, NP_001034672.1, NP_001034673.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HYNKLQADPKQ, from the internal region of the protein sequence according to NP_036539.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Colon and Prostate. Recommended concentration: 5 μg/mL.

Western Blot: Approx 50 kDa band observed in Mouse Brain lysates (calculated MW of 46.6 kDa according to NP_036539.1, NP_001034672.1and NP_001034673.1). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PICK1

Target: PICK1 (Protein Interacting With Protein Kinase C, alpha 1 (PICK1))

Alternative Name: PICK1

Background: PICK1, protein interacting with PRKCA 1, MGC15204, PRKCABP , OTTHUMP00000028509, PRKCA-binding protein, protein interacting with C kinase 1, protein kinase C, alpha binding protein

Gene ID: 9463, 18693, 84591

NCBI Accession: NP_036539