GFAP antibody (C-Term)

Catalog No. ABIN185730

This anti-GFAP antibody is a Goat Polyclonal antibody detecting GFAP in WB and ELISA. Suitable for Human, Rat and Mouse.

Quick Overview for GFAP antibody (C-Term) (ABIN185730)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivity: Human, Rat, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This GFAP antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-GFAP Antibody

Binding Specificity: C-Term

Purpose: GFAP

Sequence: DGEVIKESKQ EHKD

Specificity: GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells but its exact function remains poorly understood, despite the number of studies using it as a cell marker.

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DGEVIKESKQEHKD, from the C Terminus of the protein sequence according to NP_002046.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 48 kDa band observed in Human Cerebellum and in Mouse Brain lysates and approx.. 50 kDa in Rat Brain lysates ,(calculated MW of 49.9 kDa according to, Human NP_002046.1, Mouse NP_034407.2 and Rat NP_058705.2). Recommended concentration:

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: GFAP

Background: GFAP, glial fibrillary acidic protein , FLJ45472 , intermediate filament protein

Gene ID: 2670, 14580, 24387

NCBI Accession: NP_002046