Gelsolin antibody (Internal Region)

Catalog No. ABIN185735

The Goat Polyclonal anti-Gelsolin antibody has been validated for WB, ELISA and FACS. It is suitable to detect Gelsolin in samples from Human.

Quick Overview for Gelsolin antibody (Internal Region) (ABIN185735)

Target: Gelsolin (GSN)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Gelsolin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Verified

Product Details anti-Gelsolin Antibody

Binding Specificity: Internal Region

Purpose: Gelsolin / GSN

Sequence: PRLKDKKMDA HP

Specificity: This antibody is expected to recognize isoforms a, b, c, d, e, f, g, h

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-PRLKDKKMDAHP, from the internal region of the protein sequence according to NP_000168.1, NP_937895.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 90 kDa observed in Human Colorectal cancer lysates and in prelominary testing of Human Spleen and Parathyroid lysate (calculated MW of 85.7 kDa according to NP_000168.1). Recommended concentration: 0.1-2 μg/mL. Primary incubation 1 ho

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Gelsolin

Target: Gelsolin (GSN)

Alternative Name: GSN

Background: GSN, , gelsolin, gelsolin (amyloidosis, Finnish type) , DKFZp313L0718

Gene ID: 2934, 227753, 296654

NCBI Accession: NP_000168, NP_937895, NP_001121138, NP_001244958, NP_001244959, NP_001121135, NP_001340005, NP_001340007

Pathways: Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization, Autophagy