FEZ1 antibody (Internal Region)

Catalog No. ABIN185737

This Goat Polyclonal antibody specifically detects FEZ1 in WB, ELISA and IHC. It exhibits reactivity toward Human and Rat.

Quick Overview for FEZ1 antibody (Internal Region) (ABIN185737)

Target: FEZ1 (Fasciculation and Elongation Protein zeta 1 (Zygin I) (FEZ1))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This FEZ1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-FEZ1 Antibody

Binding Specificity: Internal Region

Purpose: FEZ1 / Zygin 1

Sequence: QNKQKEQREL MKK

Specificity: This antibody is expected to recognize isoform 1 (NP_005094.1) only.

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QNKQKEQRELMKK, from the internal region of the protein sequence according to NP_005094.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5-8 μg/mL.

Western Blot: Approx 75 kDa band observed in lysates of cell line Kelly, ans in Rat Brain lysates (calculated MW of 45.1 kDa according to human NP_005094.1 and 45.2 kDa according to Rat NP_112328.1). This molecular weight is routinely observed by other sour

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for FEZ1

Target: FEZ1 (Fasciculation and Elongation Protein zeta 1 (Zygin I) (FEZ1))

Alternative Name: FEZ1

Background: FEZ1, zygin 1, fasciculation and elongation protein zeta 1 (zygin I)

Gene ID: 9638, 235180, 81730

NCBI Accession: NP_005094