CD8 alpha antibody (AA 26-236)

Catalog No. ABIN1858316

The Rabbit Polyclonal anti-CD8 alpha antibody has been validated for IHC, WB, IP and ICC. It is suitable to detect CD8 alpha in samples from Rat.

Quick Overview for CD8 alpha antibody (AA 26-236) (ABIN1858316)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD8 alpha antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-CD8 alpha Antibody

Binding Specificity: AA 26-236

Purpose: Polyclonal Antibody to Cluster Of Differentiation 8a (CD8a)

Specificity: The antibody is a rabbit polyclonal antibody raised against CD8a. It has been selected for its ability to recognize CD8a in immunohistochemical staining and western blotting.

Cross-Reactivity: Human

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPB099Ra01Recombinant Cluster Of Differentiation 8a (CD8a)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-5 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: CD8a

Background: P32, CD8-A, Leu2, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

Pathways: TCR Signaling