CRP antibody (AA 20-222) (Biotin)

Catalog No. ABIN1858502

The Mouse Monoclonal anti-CRP antibody has been validated for WB, IHC and ICC. It is suitable to detect CRP in samples from Pig.

Quick Overview for CRP antibody (AA 20-222) (Biotin) (ABIN1858502)

Target: CRP (C-Reactive Protein (CRP))

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CRP antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-CRP Antibody (Biotin)

Binding Specificity: AA 20-222

Purpose: Biotin-Linked Monoclonal Antibody to C Reactive Protein (CRP)

Sequence: MGHHHHHHSG SEF-Q TDMIGKAFVF PKESENSYVS LTARLTKPLT AFTVCLRVYT DLNRDYSLFS YATKTQYNEI LLFRGKTAVY SISVGGADVV FKPHQSSEPM HFCMTWESTS GITELWVDGK PMVRRSLKRG YSLGTQASII LGQEQDAFAG GFEKNQCLVG DIGDVNMWDY VLSPEEINTV YAGGTFSPNV LNWRALRYEM SGEVYVKPQL WP

Specificity: The antibody is a mouse monoclonal antibody raised against CRP. It has been selected for its ability to recognize CRP in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant CRP expressed in E.coli.
The antibody is a mouse monoclonal antibody raised against CRP conjugated to biotin.

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:500-5000 Immunohistochemistry: 5-20 μg/mL,1:50-200 Immunocytochemistry: 5-20 μg/mL,1:50-200 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CRP

Target: CRP (C-Reactive Protein (CRP))

Alternative Name: C Reactive Protein

Background: C-RP, PTX1, Pentraxin-Related, Pentraxin 1

Pathways: Carbohydrate Homeostasis