FZD10 antibody (AA 235-495)

Catalog No. ABIN1858934

The Rabbit Polyclonal anti-FZD10 antibody has been validated for WB, IHC, ICC and IP. It is suitable to detect FZD10 in samples from Mouse.

Quick Overview for FZD10 antibody (AA 235-495) (ABIN1858934)

Target: FZD10 (Frizzled Family Receptor 10 (FZD10))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FZD10 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-FZD10 Antibody

Binding Specificity: AA 235-495

Purpose: Polyclonal Antibody to Frizzled Homolog 10 (FZD10)

Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-WSVLCF FSSAFTVLTF LIDPSRFRYP ERPIIFLSMC YCVYSVGYII RLFAGAESIA CDRDSGQLYV IQEGLESTGC TLVFLVLYYF GMASSLWWVV LTLTWFLAAG KKWGHEAIEA NSSYFHLAAW AIPAVKTILI LVMRRVAGDE LTGVCYVGSM DVNALTGFVL VPLACYLVIG TSFILSGFVA LFHIRRVMKT GGENTDKLEK LMVRIGVFSL LYTVPATCVI ACYFYERLNM DYWKMLATQH KCKMNNQTKT PDCLM

Specificity: The antibody is a rabbit polyclonal antibody raised against FZD10. It has been selected for its ability to recognize FZD10 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: FZD10 (AA 235-495)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for FZD10

Target: FZD10 (Frizzled Family Receptor 10 (FZD10))

Alternative Name: FZD10

Background: CD350, FZ-10, FzE7, HFz10

Pathways: WNT Signaling