ITGA5 antibody (AA 895-1049)

Catalog No. ABIN1859488

This Rabbit Polyclonal antibody specifically detects ITGA5 in WB, IHC, IP and ICC. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for ITGA5 antibody (AA 895-1049) (ABIN1859488)

Target: ITGA5 (Integrin, alpha 5 (ITGA5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITGA5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-ITGA5 Antibody

Binding Specificity: AA 895-1049

Purpose: Polyclonal Antibody to Integrin Alpha 5 (ITGa5)

Specificity: The antibody is a rabbit polyclonal antibody raised against ITGa5. It has been selected for its ability to recognize ITGa5 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Integrin Alpha 5 (ITGa5) corresdonding to Glu895~Ala1049 with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.22 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: ProClin, Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

References for anti-ITGA5 antibody (ABIN1859488)

Hoshiba, Maruyama, Sato, Endo, Kawazoe, Chen, Tanaka: "Maintenance of Cartilaginous Gene Expression of Serially Subcultured Chondrocytes on Poly(2-Methoxyethyl Acrylate) Analogous Polymers." in: Macromolecular bioscience, Vol. 17, Issue 12, (2018) (PubMed).

Target Details for ITGA5

Target: ITGA5 (Integrin, alpha 5 (ITGA5))

Alternative Name: ITGa5

Background: CD49e, CD49-E, ITG-A5, FNRA, VLA5A, Fibronectin Receptor,Alpha Polypeptide, Integrin alpha-F, CD49 antigen-like family member E

Pathways: Integrin Complex