ENO3 antibody (AA 3-434) (APC)

Catalog No. ABIN1859884

This Guinea Pig Polyclonal antibody specifically detects ENO3 in WB, IHC, ICC and IP. It exhibits reactivity toward Rabbit.

Quick Overview for ENO3 antibody (AA 3-434) (APC) (ABIN1859884)

Target: ENO3 (Enolase 3 (Beta, Muscle) (ENO3))

Reactivity: Rabbit

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This ENO3 antibody is conjugated to APC

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-ENO3 Antibody (APC)

Binding Specificity: AA 3-434

Purpose: Polyclonal Antibody to Enolase 3 (ENO3)

Sequence: MGHHHHHHSG SEF-MAMQKIFARE ILDSRGNPTV EVDLHTAKGR FRAAVPSGAS TGIYEALELR DGDKSRYLGK GVLKAVEHIN KTLGPALLEK KLSVVDQEKV DKFMIELDGT ENKSKFGANA ILGVSLAVCK AGAAEKGVPL YRHIADLAGN HDLVLPVPAF NVINGGSHAG NKLAMQEFMI LPVGASSFRE AMRIGAEVYH HLKGVIKAKY GKDATNVGDE GGFAPNILEN NEALELLKTA IQAAGYPDKV VIGMDVAASE FHRNGKYDLD FKSPDDPARH ITGQKLGELY KSFIKNYPVV SIEDPFDQDD WATWTSFLSG VDIQIVGDDL TVTNPKRIAQ AVEKKACNCL LLKVNQIGSV TESIQACKLA QSNGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL MRIEEALGDK AVFAGRKFRN PKAK

Specificity: The antibody is a cavia polyclonal antibody raised against ENO3. It has been selected for its ability to recognize ENO3 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPB433Rb01Recombinant Enolase 3 (ENO3)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ENO3

Target: ENO3 (Enolase 3 (Beta, Muscle) (ENO3))

Alternative Name: ENO3

Background: MSE, Beta Enolase, Enolase 3, 2-phospho-D-glycerate hydro-lyase, Skeletal muscle enolase