NOS2 antibody (AA 868-909)

Catalog No. ABIN1860032

The Rabbit Polyclonal anti-NOS2 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect NOS2 in samples from Mouse.

Quick Overview for NOS2 antibody (AA 868-909) (ABIN1860032)

Target: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOS2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-NOS2 Antibody

Binding Specificity: AA 868-909

Purpose: Polyclonal Antibody to Nitric Oxide Synthase 2, Inducible (NOS2)

Specificity: The antibody is a rabbit polyclonal antibody raised against NOS2. It has been selected for its ability to recognize NOS2 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPA837Mu03Recombinant Nitric Oxide Synthase 2, Inducible (NOS2)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: ProClin, Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NOS2

Target: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Alternative Name: NOS2

Background: NOS2A, INOS, HEP-NOS, I-NOS, Hepatocytes Oxide Synthase, Peptidyl-cysteine S-nitrosylase NOS2

Pathways: Retinoic Acid Receptor Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Inositol Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process