SP1 antibody (AA 260-591)

Catalog No. ABIN1860600

This anti-SP1 antibody is a Rabbit Polyclonal antibody detecting SP1 in WB, IHC, ICC and IP. Suitable for Human.

Quick Overview for SP1 antibody (AA 260-591) (ABIN1860600)

Target: SP1 (Sp1 Transcription Factor (SP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-SP1 Antibody

Binding Specificity: AA 260-591

Purpose: Polyclonal Antibody to Specificity Protein 1 (Sp1)

Specificity: The antibody is a rabbit polyclonal antibody raised against Sp1. It has been selected for its ability to recognize Sp1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPM264Hu01Recombinant Specificity Protein 1 (Sp1)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SP1

Target: SP1 (Sp1 Transcription Factor (SP1))

Alternative Name: Specificity Protein 1

Background: TSFP1, Sp1 Transcription Factor

Pathways: Regulation of Lipid Metabolism by PPARalpha, Myometrial Relaxation and Contraction