TGM1 antibody (AA 433-692) (APC)

Catalog No. ABIN1860740

The Guinea Pig Polyclonal anti-TGM1 antibody has been validated for WB, IHC, ICC and IP. It is suitable to detect TGM1 in samples from Rabbit.

Quick Overview for TGM1 antibody (AA 433-692) (APC) (ABIN1860740)

Target: TGM1 (Transglutaminase 1, Keratinocyte (TGM1))

Reactivity: Rabbit

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This TGM1 antibody is conjugated to APC

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-TGM1 Antibody (APC)

Binding Specificity: AA 433-692

Purpose: Polyclonal Antibody to Transglutaminase 1 (TGM1)

Sequence: MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQ GPLGSEF- MDIYFDEN MKPLEHLNRD SVWNFHVWND CWMKRPDLPS GFDGWQVVDA TPQETSSGIF CCGPCSVESV KNGLVYMKYD TPFIFAEVNS DKVYWQRQDD GSFKIVYVEE KAIGTLIVTK AVRSHMREDI THIYKHPEGS DAERKAVETA AAHGSKPNVY DSRDSAEDVA MQVEAQDAVM GQDLTVSVVL TNRSSSRRTV KLHLYLSVTF YTGVTGSIFK ESKKEVVLAA GSSDSVVMPV AYKEYRPHLV DQGAMLLNVS GH

Specificity: The antibody is a cavia polyclonal antibody raised against TGM1. It has been selected for its ability to recognize TGM1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant TGM1 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against TGM1.

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for TGM1

Target: TGM1 (Transglutaminase 1, Keratinocyte (TGM1))

Alternative Name: TGM1

Background: TGK, ICR2, KTG, LI, LI1, TGASE, Transglutaminase 1, Keratinocyte, Transglutaminase K, K Polypeptide Epidermal Type I, Protein-Glutamine-Gamma-Glutamyltransferase