Lipocalin 2 antibody (AA 23-199)

Catalog No. ABIN1862968

This Rabbit Polyclonal antibody specifically detects Lipocalin 2 in WB, IHC, IP and ICC. It exhibits reactivity toward Pigand has been mentioned in 1 publication.

Quick Overview for Lipocalin 2 antibody (AA 23-199) (ABIN1862968)

Target: Lipocalin 2 (LCN2)

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Lipocalin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Lipocalin 2 Antibody

Binding Specificity: AA 23-199

Purpose: Polyclonal Antibody to Neutrophil gelatinase-associated lipocalin (NGAL)

Specificity: The antibody is a rabbit polyclonal antibody raised against NGAL. It has been selected for its ability to recognize NGAL in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPB388Po01Recombinant Neutrophil gelatinaseassociated lipocalin (NGAL)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-Lipocalin 2 antibody (ABIN1862968)

Davidson, Khailova, Treece, Robison, Soranno, Jaggers, Ing, Lawson, Lujan: "Alkaline Phosphatase Treatment of Acute Kidney Injury in an Infant Piglet Model of Cardiopulmonary Bypass with Deep Hypothermic Circulatory Arrest." in: Scientific reports, Vol. 9, Issue 1, pp. 14175, (2020) (PubMed).

Target Details for Lipocalin 2

Target: Lipocalin 2 (LCN2)

Alternative Name: NGAL

Background: Lipocalin-2, LCN2, p25, Lipocalin 2, Oncogene 24p3, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, Siderocalin LCN2

Pathways: Cellular Response to Molecule of Bacterial Origin, Transition Metal Ion Homeostasis