AF4 antibody (AA 1-183)

Catalog No. ABIN1866575

The Rabbit Polyclonal anti-AF4 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect AF4 in samples from Human.

Quick Overview for AF4 antibody (AA 1-183) (ABIN1866575)

Target: AF4 (AFF1) (AF4/FMR2 Family, Member 1 (AFF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AF4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-AF4 Antibody

Binding Specificity: AA 1-183

Purpose: Polyclonal Antibody to AF4/FMR2 Family, Member 1 (AFF1)

Specificity: The antibody is a rabbit polyclonal antibody raised against AFF1. It has been selected for its ability to recognize AFF1 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPJ872Hu01Recombinant AF4/FMR2 Family, Member 1 (AFF1)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunofluorescence:5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.31 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: ProClin, Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for AF4

Target: AF4 (AFF1) (AF4/FMR2 Family, Member 1 (AFF1))

Alternative Name: AFF1

Background: AF-4, AF4, AF4-MLL, MLL/AF4, MLLT2, PBM1, Myeloid/Lymphoid Or Mixed-Lineage Leukemia, Translocated To 2, Pre-B-Cell Monocytic Leukemia Partner 1