C4B antibody (FITC)
Quick Overview for C4B antibody (FITC) (ABIN1866969)
Target
See all C4B (C4b) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Purpose
- FITC-Linked Polyclonal Antibody to Complement C4-B (C4B)
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Specificity
- The antibody is a rabbit polyclonal antibody raised against C4B. It has been selected for its ability to recognize C4B in immunohistochemical staining and western blotting.
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Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
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Immunogen
- The antibody is a rabbit polyclonal antibody raised against C4b conjugated to fitc.
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Isotype
- IgG
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Application Notes
- Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.
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Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 500 μg/mL
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Buffer
- PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
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Preservative
- Sodium azide
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Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
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Handling Advice
- Avoid repeated freeze/thaw cycles
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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Expiry Date
- 12 months
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- C4B (C4b) (Complement Component C4b (C4b))
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Alternative Name
- Complement C4-B
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Background
- CO4, CPAMD3, Basic complement C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3, Complement component 4B, Chido blood group
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Pathways
- Complement System
Target
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