DDIT3 antibody (AA 1-168)

Catalog No. ABIN1867534

This Rabbit Polyclonal antibody specifically detects DDIT3 in WB, IHC, IP and ICC. It exhibits reactivity toward Mouse.

Quick Overview for DDIT3 antibody (AA 1-168) (ABIN1867534)

Target: DDIT3 (DNA-Damage-Inducible Transcript 3 (DDIT3))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDIT3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-DDIT3 Antibody

Binding Specificity: AA 1-168

Purpose: Polyclonal Antibody to DNA Damage Inducible Transcript 3 (DDIT3)

Specificity: The antibody is a rabbit polyclonal antibody raised against DDIT3. It has been selected for its ability to recognize DDIT3 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPJ282Mu01Recombinant DNA Damage Inducible Transcript 3 (DDIT3)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mLImmunohistochemistry: 5-20 μg/mLImmunocytochemistry: 5-20 μg/mLOptimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: ProClin, Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for DDIT3

Target: DDIT3 (DNA-Damage-Inducible Transcript 3 (DDIT3))

Alternative Name: DDIT3

Background: CEBPZ, CHOP, CHOP10, GADD153, C/EBP-homologous protein 10, CCAAT/enhancer-binding protein homologous protein, Growth arrest and DNA damage-inducible GADD153

Pathways: Regulation of Muscle Cell Differentiation, ER-Nucleus Signaling, Skeletal Muscle Fiber Development, Cell RedoxHomeostasis