Neurofilament H (200 kDa) (phosphospecific) antibody

Catalog No. ABIN187214

The Mouse Monoclonal anti- antibody has been validated for WB and IF. It is suitable to detect in samples from Chicken and Mammalian.

Quick Overview for Neurofilament H (200 kDa) (phosphospecific) antibody (ABIN187214)

Target: Neurofilament H (200 kDa)

Reactivity: Chicken, Mammalian

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: NAP4

Product Details

Binding Specificity: phosphospecific

Specificity: Species Reactivity: Mammals, Chicken.

Immunogen: Mice were immunized with purified pig spinal cord NF-H.

Isotype: IgG1

Application Details

Application Notes: Immunocytochemistry: Use at 1:100 - 1:500 Immunofluorescence: Use at 1:100 - 1:500 Western blot: Use at 1:1,000 - 1:2,500 The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 µl with 50% glycerol, 0.01% thimerosal and 1.0 mg/ml BSA.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details

Target: Neurofilament H (200 kDa)

Background: Neurofilaments are members of the intermediate filament family and are neuron-specific. There are three major proteins, NF-H, NF-L and NF-M. Accumulation of neurofilaments is observed during the progression of numerous neurological diseases. This antibody recognizes phosphorylated NF-H sequence n-KSP-c (lysine-serine-proline). Some cross-reactivity to the phosphorylated n-KSP-c of subunit NF-M may be observed in some species.