Neurofilament H (200 kDa) (phosphospecific) antibody
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- Target
- Neurofilament H (200 kDa)
- Binding Specificity
- phosphospecific
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Reactivity
- Chicken, Mammalian
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF)
- Specificity
- Species Reactivity: Mammals, Chicken.
- Immunogen
- Mice were immunized with purified pig spinal cord NF-H.
- Clone
- NAP4
- Isotype
- IgG1
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- Application Notes
- Immunocytochemistry: Use at 1:100 - 1:500 Immunofluorescence: Use at 1:100 - 1:500 Western blot: Use at 1:1,000 - 1:2,500 The optimal dilution for a specific application should be determined by the researcher.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 µl with 50% glycerol, 0.01% thimerosal and 1.0 mg/ml BSA.
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
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- Target
- Neurofilament H (200 kDa)
- Background
- Neurofilaments are members of the intermediate filament family and are neuron-specific. There are three major proteins, NF-H, NF-L and NF-M. Accumulation of neurofilaments is observed during the progression of numerous neurological diseases. This antibody recognizes phosphorylated NF-H sequence n-KSP-c (lysine-serine-proline). Some cross-reactivity to the phosphorylated n-KSP-c of subunit NF-M may be observed in some species.
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