Thymine Dimer antibody

Catalog No. ABIN187263

The Mouse Monoclonal anti-Thymine Dimer antibody (Clone KTM53) (ABIN187263) specifically detects Thymine Dimer in ISH. The antibody is reactive with Chemical samples.

Quick Overview for Thymine Dimer antibody (ABIN187263)

Target: Thymine Dimer

Reactivity: Chemical

Host: Mouse

Clonality: Monoclonal

Application: In situ hybridization (ISH)

Clone: KTM53

Product Details

Specificity: Reacts specifically with thymine dimers produced by UV irradiation in double- or single-stranded DNA. Does not react with (6-4) photo products.

Immunogen: UV-irradiated salmon sperm DNAMouse myeloma (P3/X63-Ag8) x immunized mouse (Balb/c) splenocytes.

Isotype: IgG1

Application Details

Application Notes: In situ hybridization: Use at a 1:40 to 1:80 dilution. The dilution factor depends on the application. Frozen sections of fixed tissue or formalin-fixed, paraffin-embedded sections are recommended. Southern, Northern and Dot Blot: For the detection of thymine dimers in UV-damaged DNA and thymine-dimerized DNA probes used for in situ. (reported to work, but no data is available) Immunocytochemistry: Reported to work by end-user, protocol available. Immunohistochemistry: Published by user. The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 200 µL of 0.5 mg/mL purified monoclonal antibody in PBS with protein stabilizer and 0.1 % sodium azide. Purified by Protein A chromatography.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details

Target: Thymine Dimer

Target Type: Chemical