MVP antibody

Catalog No. ABIN187371

The Mouse Monoclonal anti-MVP antibody (Clone 5J2) (ABIN187371) specifically detects MVP in WB and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for MVP antibody (ABIN187371)

Target: MVP (Major Vault Protein (MVP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MVP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 5J2

Product Details anti-MVP Antibody

Specificity: The mAb 1011 is specific for a 104 kDa protein. This mAb is one of four mAb which recognize different epitopes of the protein. This 104 kDa protein is the major vault protein and has shown to interact with the estrogen receptor. The protein is part of a very large vault ribonucleoprotein complex present in all eukaryotic cells and its structure and protein composition is highly conserved. Because of the size, shape, and protein and RNA composition of this complex the particles are different from other ribonucleoproteins. Species Reactivity: Human, others not tested.

Immunogen: Recombinant human MVPA BALB/c mouse was immunized with 5 µg of affinity purified nuclear extract proteins. Spleen cells were fused with equal number of Sp0Ag-14 myeloma cells. Cells whose supernatant showed a positive signal for a 104 kDa band were selected and cloned by limited dilution.

Isotype: IgG1

Application Details

Application Notes: The mAb 1011 can be applied for the detection of the major vault protein in a large number of eukaryotic cells including the MCF-7 and Hela tumor cell lines. The mAb are especially applicable to study the association of estrogen receptor with vaults and the study of the mechanism of action of estrogenic hormones. Western blot Immunohistology (Frozen) The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Handling

Buffer: 100 µg purified antibody.

Storage: 4 °C

Target Details for MVP

Target: MVP (Major Vault Protein (MVP))

Alternative Name: Major Vault Protein