MVP antibody

Catalog No. ABIN187372

This Mouse Monoclonal antibody specifically detects MVP in WB and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for MVP antibody (ABIN187372)

Target: MVP (Major Vault Protein (MVP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MVP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 1027

Product Details anti-MVP Antibody

Specificity: Species Reactivity: Human, other species not tested.

Immunogen: A BALB/c mouse was immunized with 5 µg of affinity purified nuclear extract proteins. Spleen cells were fused with equal number of Sp0Ag-14 myeloma cells. Cells whose supernatant showed a positive signal for a 104 kDa band were selected and cloned by limited dilution.

Isotype: IgM

Application Details

Application Notes: Clone 1027 can be applied for the detection of MVP/LRP in a large number of eukaryotic cells including the MCF-7 and Hela tumor cell lines. Immunohistochemistry: Frozen sections Western blot The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 100 µg purified, lyophilized.

Storage: 4 °C

Target Details for MVP

Target: MVP (Major Vault Protein (MVP))

Alternative Name: Major Vault Protein

Background: The mAb clone 1027 is specific for a 104 kDa protein. The antibody is one of four mAb which recognize different epitopes of the protein. This 104 kDa protein is the major vault protein (MVP) also described as the lung resistance protein (LRP) and has been shown to interact with the estrogen receptor. The protein is part of a very large vault ribonucleoprotein complex present in all eukaryotic cells and its structure and protein composition is highly conserved. Because of the size, shape, protein and RNA composition of this complex the particles are different from other ribonucleoproteins. The mAb is especially applicable for studying the association of estrgen receptor with vaults and the study of the mechanism of action of estrogenic hormones.