S100A9 antibody

Catalog No. ABIN187527

The Mouse Monoclonal anti-S100A9 antibody (Clone 47-8D3) (ABIN187527) specifically detects S100A9 in WB, IHC (p), EIA and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for S100A9 antibody (ABIN187527)

Target: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This S100A9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 47-8D3

Product Details anti-S100A9 Antibody

Specificity: Anti-MRP-14 clone 47-8D3 reacts with macrophages and detects the well-known leukocyte L1, cystic fibrosis antigen. Using Western blot analysis 47-8D3 was found to detect a single protein band of 14 kDa in lysates of human monocytes and granulocytes, which was demonstrated to be the calcium-binding protein MRP14. Species Reactivity: Human. Others not tested.

Immunogen: A BALB/c mouse was immunized with human peripheral blood monocyte components. Splenocytes were fused with mouse myeloma cell line NS-1.

Isotype: IgG1

Application Details

Application Notes: Western blot Immunohistochemistry (Frozen and paraffin) Staining strongly enhanced after digestion with Trypsin). ELISA This antibody may be used for phenotypical characterization of macrophages and myelomonocytic cells in situ. The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Handling

Buffer: 100 µg purified monoclonal antibody.

Storage: 4 °C

Target Details for S100A9

Target: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Alternative Name: S100A9 / Calgranulin-B / MRP14

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins