Aggrecan antibody

Catalog No. ABIN187628

The Mouse Monoclonal anti-Aggrecan antibody is suitable to detect Aggrecan in samples from Human. It has been validated for WB, IP, IHC (p) and EIA.

Quick Overview for Aggrecan antibody (ABIN187628)

Target: Aggrecan (ACAN)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Aggrecan antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: 6F4

Product Details anti-Aggrecan Antibody

Specificity: Reacts with chondroitin sulfate proteoglycan from cartilage (aggrecan). Species Reactivity: Reacts with human. No crossreactivity with bovine nasal cartilage, rat chondrosarcoma, chick or shark cartilage aggrecan. Other species not tested. Controls: Purified antigen, cartilage extracts or mature cultured chondrocytes.

Immunogen: Human large molecular weight proteoglycan of articular cartilage (aggrecan).

Isotype: IgG1

Application Details

Application Notes: ELISA: 1:500-1:1,000 (highest ELISA reactivity of our 3 anti-chondroitin/keratan sufate proteoglycans). In solid phase immunoassays, endo-beta-galactosidase digestion of aggrecan increases the immunoreactivity of the antibody. Also reacts with papain-digested aggrecan. Immunohistochemistry: (paraffin embedded): 1:50-1:3,000. Treat tissue sections with 2 mg/ml testicular hyaluronidase in 50 mM Na-acetate buffer, pH 5.5 for 1 hr at 37C, followed by treatment with 0.2 mg/ml Protease XXIV in TBS, pH 7.2, for the same time and temperature. Immunoprecipitation: 1:50-1:300 Western blotting: 1:100-1:300 Reacts with 3 trypsin-generated fragments of human cartilage aggrecan (prior digested with chondroitinase and endo-beta-galactosidase) which have apparent MW of 55, 65 and 95 kDa. The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 µl ascites containing 0.01% sodium azide. Antibody concentration >1 mg/ml.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for Aggrecan

Target: Aggrecan (ACAN)

Alternative Name: Aggrecan

Pathways: Glycosaminoglycan Metabolic Process, Dicarboxylic Acid Transport