pan Keratin antibody

Catalog No. ABIN187832

The Rabbit Polyclonal anti-pan Keratin antibody has been validated for IHC (p) and IHC (fro). It is suitable to detect pan Keratin in samples from Human, Rat, Mouse, Pig, Dog, Rabbit, Hamster and Avian.

Quick Overview for pan Keratin antibody (ABIN187832)

Target: pan Keratin (panKRT)

Reactivity: Human, Rat, Mouse, Pig, Dog, Rabbit, Hamster, Avian

Host: Rabbit

Clonality: Polyclonal

Conjugate: This pan Keratin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-pan Keratin Antibody

Specificity: Recognizes keratin Species Reactivity: Most mammals and birds.

Immunogen: Keratin extracted from human skin. Antigen Location: Cytoplasm

Application Details

Application Notes: Immunohistochemistry - Frozen sections: Fixation for 3 min. with 1% formaldehyde is recommended. Formaldehyde-fixed, paraffin-embedded tissue sections: Proteolytic pretreatment with pronase or trypsine is recommended. Use at a 1:50 dilution in PBS or Tris. Recommended positive control: Squamous cell carcinoma, skin, cervix. The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.25 mL affinity chromatography purified liquid antiserum.

Storage: 4 °C

Target Details for pan Keratin

Target: pan Keratin (panKRT)

Alternative Name: Keratin

Background: This polyclonal antibody reacts with keratin of most mammals and birds. A positive reaction is obtained with all stratified squamous epithelia and in epidermal appendages such as hairfollicles and sebaceous glands. Also the epithelia of the urinary tract, stomach, small and large intestine, uterine endometrium, bile ducts, prostate, kidney collecting tubules, thymic reticulum cells, mesothelium and to a lesser extent hepatocytes, adenocarcinomas, transitional cell carcinomas and anaplastic carcinomas can be identified by this antiserum. Renal cell adenocarcinomas and small cell anaplastic lung tumors may remain negative when examined in paraffin sections. Hepatocytes and pancreatic acini show a positive staining. Nearly all cultured epithelial cells are positive, all non-epithelial cells are negative.