PARP1 antibody (pThr373)

Catalog No. ABIN1881631

This Rabbit Polyclonal antibody specifically detects PARP1 in DB. It exhibits reactivity toward Human and has been mentioned in 4+ publications.

Quick Overview for PARP1 antibody (pThr373) (ABIN1881631)

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PARP1 antibody is un-conjugated

Application: Dot Blot (DB)

Clone: RB40141

Product Details anti-PARP1 Antibody

Binding Specificity: pThr373

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This PARP1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T373 of human PARP1.

Isotype: Ig Fraction

Application Details

Application Notes: DB: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-PARP1 antibody (ABIN1881631)

Dong, Bey, Li, Kabbani, Yan, Xie, Hsieh, Gao, Boothman: "Prostate cancer radiosensitization through poly(ADP-Ribose) polymerase-1 hyperactivation." in: Cancer research, Vol. 70, Issue 20, pp. 8088-96, (2010) (PubMed).

Krishnakumar, Kraus: "PARP-1 regulates chromatin structure and transcription through a KDM5B-dependent pathway." in: Molecular cell, Vol. 39, Issue 5, pp. 736-49, (2010) (PubMed).

Majewski, Thurston, Ramalingam, Kiela, Ghishan: "Cooperative role of NF-{kappa}B and poly(ADP-ribose) polymerase 1 (PARP-1) in the TNF-induced inhibition of PHEX expression in osteoblasts." in: The Journal of biological chemistry, Vol. 285, Issue 45, pp. 34828-38, (2010) (PubMed).

Kim, Kang, Lee, Lee, Lee, Choi, Lee, Cho, Jin, Jheon, Son, Lee, Jung, Cha, Kim, Kang, Kam, Jung, Jheon, Park: "Comprehensive analysis of DNA repair gene polymorphisms and survival in patients with early stage non-small-cell lung cancer." in: Cancer science, Vol. 101, Issue 11, pp. 2436-42, (2010) (PubMed).

Target Details for PARP1

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Alternative Name: PARP1

Background: This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.

Molecular Weight: 113084

NCBI Accession: NP_001609

UniProt: P09874

Pathways: Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location