ATG4C antibody (C-Term)

Catalog No. ABIN1882160

The Rabbit Polyclonal anti-ATG4C antibody has been validated for WB and IHC (p). It is suitable to detect ATG4C in samples from Human and Mouse. There are 3+ publications available.

Quick Overview for ATG4C antibody (C-Term) (ABIN1882160)

Target: ATG4C (Autophagy related 4C Cysteine Peptidase (ATG4C))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG4C antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB7499

Product Details anti-ATG4C Antibody

Binding Specificity: AA 419-448, C-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This ATG4C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 419-448 amino acids from the C-terminal region of human ATG4C.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-ATG4C antibody (ABIN1882160)

Ma, Panda, Lin: "Temporal orchestration of circadian autophagy rhythm by C/EBP?." in: The EMBO journal, Vol. 30, Issue 22, pp. 4642-51, (2012) (PubMed).

Norman, Cohen, Bampton: "The in vitro cleavage of the hAtg proteins by cell death proteases." in: Autophagy, Vol. 6, Issue 8, pp. 1042-56, (2010) (PubMed).

Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, (2004) (PubMed).

Target Details for ATG4C

Target: ATG4C (Autophagy related 4C Cysteine Peptidase (ATG4C))

Alternative Name: ATG4C

Background: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4 is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.

Molecular Weight: 52497

NCBI Accession: NP_116241, NP_835739

UniProt: Q96DT6

Pathways: Autophagy