LIMK2 antibody (AA 1-262)

Catalog No. ABIN1885861

This Rabbit Polyclonal antibody specifically detects LIMK2 in WB, IHC and IF. It exhibits reactivity toward Human.

Quick Overview for LIMK2 antibody (AA 1-262) (ABIN1885861)

Target: LIMK2 (LIM Domain Kinase 2 (LIMK2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIMK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-LIMK2 Antibody

Binding Specificity: AA 1-262

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 262 of LIM kinase 2

Application Details

Application Notes: Suggested dilutions:
Western blotting: 1.500-1.3000
Immunohistochemistry: 1.100-1.250
Immunofluorescence: 1.100-1.200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.1 M Tris-buffered saline with 10 % Glycerol (pH 7.0).0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: Biohazard Informations: This product contains thimerosal which is hazardous.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for LIMK2

Target: LIMK2 (LIM Domain Kinase 2 (LIMK2))

Alternative Name: LIM kinase 2

Background: There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain.LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers.Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions.LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain.The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity.It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton.At least three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq]

Molecular Weight: 78 kDa

Gene ID: 3985

NCBI Accession: NM_001031801, NP_001026971