CLP1 antibody (AA 142-341)

Catalog No. ABIN1886042

The Rabbit Polyclonal anti-CLP1 antibody has been validated for WB, IHC and IF. It is suitable to detect CLP1 in samples from Human.

Quick Overview for CLP1 antibody (AA 142-341) (ABIN1886042)

Target: CLP1 (Cleavage and Polyadenylation Factor I Subunit 1 (CLP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CLP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CLP1 Antibody

Binding Specificity: AA 142-341

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 142 and 341 of HEAB

Application Details

Application Notes: Suggested dilutions:
Immunohistochemistry: 1.100-1.250
Immunofluorescence: 1.100-1.200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.1 M Tris-buffered saline with 20 % Glycerol (pH 7.0).0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: Biohazard Informations: This product contains thimerosal which is hazardous.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for CLP1

Target: CLP1 (Cleavage and Polyadenylation Factor I Subunit 1 (CLP1))

Alternative Name: HEAB

Background: Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids.dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component.Appears to have roles in both tRNA splicing and mRNA 3'-end formation.Component of the tRNA splicing endonuclease complex.Phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing, this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA.Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation.Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC).However endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.

Molecular Weight: 48 kDa

Gene ID: 10978

Pathways: Ribonucleoprotein Complex Subunit Organization