CLP1 antibody (C-Term)

Catalog No. ABIN1537107

The Rabbit Polyclonal anti-CLP1 antibody (Clone RB37304) (ABIN1537107) specifically detects CLP1 in WB. The antibody is reactive with Human samples.

Quick Overview for CLP1 antibody (C-Term) (ABIN1537107)

Target: CLP1 (Cleavage and Polyadenylation Factor I Subunit 1 (CLP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CLP1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB37304

Product Details anti-CLP1 Antibody

Binding Specificity: AA 308-336, C-Term

Predicted Reactivity: B, C, Pr, M, Rat

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This CLP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 308-336 amino acids from the C-terminal region of human CLP1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: CLP1 Antibody (C-term) can be refrigerated at 2-8 °C for up to 6 months. For long term storage, keep at -20 °C.

Expiry Date: 6 months

Target Details for CLP1

Target: CLP1 (Cleavage and Polyadenylation Factor I Subunit 1 (CLP1))

Alternative Name: CLP1

Background: Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Appears to have roles in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex. Phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing, this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.

Molecular Weight: 47646

Gene ID: 10978

NCBI Accession: NP_001136069, NP_006822

UniProt: Q92989

Pathways: Ribonucleoprotein Complex Subunit Organization