WT1 antibody (AA 277-495)

Catalog No. ABIN1886304

This anti-WT1 antibody is a Rabbit Polyclonal antibody detecting WT1 in IHC, IF and WB. Suitable for Human.

Quick Overview for WT1 antibody (AA 277-495) (ABIN1886304)

Target: WT1 (Wilms Tumor 1 (WT1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WT1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blotting (WB)

Product Details anti-WT1 Antibody

Binding Specificity: AA 277-495

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 277 and 495 (P19544) of Wilms Tumor 1

Application Details

Application Notes: Suggested dilutions:
Western blotting: 1.500-1.3000
Immunohistochemistry: 1.50-1.500
Immunofluorescence: 1.100-1.200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.1 M Tris-buffered saline with 10 % Glycerol (pH 7.0).0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: Biohazard Informations: This product contains thimerosal which is hazardous.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Alternative Name: WT1

Background: This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus.It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors.Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized.There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms.Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.[provided by RefSeq]

Molecular Weight: 56 kDa

Gene ID: 7490

NCBI Accession: NM_024426, NP_077744

Pathways: Tube Formation