NAPA antibody (AA 78-269)

Catalog No. ABIN1886662

This Rabbit Polyclonal antibody specifically detects NAPA in WB and IF. It exhibits reactivity toward Human.

Quick Overview for NAPA antibody (AA 78-269) (ABIN1886662)

Target: NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NAPA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-NAPA Antibody

Binding Specificity: AA 78-269

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 78 and 269 of Human NAPA

Application Details

Application Notes: Suggested dilutions:
Western blotting: 1.500-1.3000
Immunofluorescence: 1.100-1.200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.1 M Tris-buffered saline with 10 % Glycerol (pH 7.0).0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: Biohazard Informations: This product contains thimerosal which is hazardous.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for NAPA

Target: NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))

Alternative Name: alpha SNAP

Background: The 'SNARE hypothesis' is a model explaining the process of docking and fusion of vesicles to their target membranes.According to this model, membrane proteins from the vesicle (v-SNAREs) and proteins from the target membrane (t-SNAREs) govern theof vesicle targeting and docking through mutual recognition.Once the 2 classes of SNAREs bind to each other, they form a complex that recruits the general elements of the fusion apparatus, namely NSF (N-ethylmaleimide-sensitive factor) and SNAPs (soluble NSF-attachment proteins), to the site of membrane fusion, thereby forming the 20S fusion complex.Alpha- and gamma-SNAP are found in a wide range of tissues and act synergistically in intra-Golgi transport.The sequence of the predicted 295-amino acid human protein encoded by NAPA shares 37%, 60%, and 67% identity with the sequences of yeast, Drosophila, and squid alpha-SNAP, respectively.Platelets contain some of the same proteins, including NSF, p115/TAP, alpha-SNAP, gamma-SNAP, and the t-SNAREs syntaxin-2 and syntaxin-4, that are used in many vesicular transport processes in other cell types.Platelet exocytosis uses a molecular mechanism similar to that used by other secretory cells, such as neurons, although the proteins used by the platelet and their modes of regulation may be quite different.[provided by RefSeq]

Molecular Weight: 33 kDa

Gene ID: 8775

NCBI Accession: NM_003827, NP_003818

Pathways: Synaptic Vesicle Exocytosis, Asymmetric Protein Localization