BUB1 antibody (C-Term)

Catalog No. ABIN1887318

The Rabbit Polyclonal anti-BUB1 antibody (ABIN1887318) specifically detects BUB1 in WB, ELISA and ICC. The antibody is reactive with Human and Rat samples.

Quick Overview for BUB1 antibody (C-Term) (ABIN1887318)

Target: BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BUB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Product Details anti-BUB1 Antibody

Binding Specificity: C-Term

Purification: Affinity chromatography purified via peptide column

Immunogen: 22 amino acid peptide from near the carboxy terminus of human Bub1.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid freezing and thawing repeatly.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term use.Store at -20 °C for long term preservation.

Target Details for BUB1

Target: BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))

Alternative Name: Bub1

Background: Bub1 was initially identified as the mammalian homolog to the yeast protein through a cDNA screen.Bub1 encodes a kinase involved in the formation of the spindle checkpoint, an important mechanism that ensures high fidelity mitotic chromosome segregation.It is thought to be required for assembly of a functional inner centromere, sister chromatid cohesion via targeting of the Shugoshim protein and metaphase congression.Bub1 functions by phosphorylating cdc20, a member of the mitotic checkpoint complex and activating the spindle checkpoint.A related protein kinase Bub3 interacts with Bub1 and targets it to kinetochores prior to chromosome alignment.Mutations in bub1 have been associated with aneuploidy and several forms of cancer.
Synonyms: Budding uninhibited by benzimidazoles 1

UniProt: O43683