GCLC antibody (C-Term)

Catalog No. ABIN190704

Cited in 2+ publications. The Rabbit Polyclonal anti-GCLC antibody (ABIN190704) specifically detects GCLC in WB and IL. The antibody is reactive with Zea mays, Tomato, Salicornia, Nicotiana tabacum and Arabidopsis thaliana samples.

Quick Overview for GCLC antibody (C-Term) (ABIN190704)

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Reactivity: Zea mays, Tomato, Salicornia, Nicotiana tabacum, Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCLC antibody is un-conjugated

Application: Western Blotting (WB), Immunolocalization (IL)

Product Details anti-GCLC Antibody

Binding Specificity: C-Term

Purpose: Anti-Gamma-ECS | Gamma glutamylcysteine synthase

Specificity: The antibody recognizes closely related tobacco class I isoforms: endochitinase ACHN-A (ca. 34 kDa) and endochitinase B CHN-B (ca. 32 kDa)

Cross-Reactivity (Details): Arabidopsis thaliana, Nicotiana tabcum, Salicornia sp., Solanum lycopersicum, Zea mays
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Marchantia polymorpha,Pisum sativum,Riciuns communis,Oryza sativa,Triticum aestivum

Purification: Protein G purified

Immunogen: KLH-conjugated synthetic peptide derived from C-terminal part of Zea mays gamma-ECS Q8W4W3

Isotype: IgG

Application Details

Application Notes: 1 : 5000 (IL), 1 : 5000 (WB)

Comment:

antibodies has been used in immunolocalization on Arabidopsis thaliana

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water

Buffer: Total IgG. Protein G purified in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References for anti-GCLC antibody (ABIN190704)

Sobrino-Plata, Carrasco-Gil, Abadía, Escobar, Álvarez-Fernández, Hernández: "The role of glutathione in mercury tolerance resembles its function under cadmium stress in Arabidopsis." in: Metallomics : integrated biometal science, Vol. 6, Issue 2, pp. 356-66, (2014) (PubMed).

Keefe, Hinz, Meins: "The effect of ethylene on the cell-type-specific and intracellular localization of β-1,3-glucanase and chitinase in tobacco leaves." in: Planta, Vol. 182, Issue 1, pp. 43-51, (2013) (PubMed).

Target Details for GCLC

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Alternative Name: Gamma-ECS

Background:

Background: Gamma-glutamylcysteine synthase (gamma-ECS) (EC 6.3.2.2) catalyses the first step of glutathione (GSH) synthesis, producing gamma-glutamylcysteine (gamma-EC) from L-glutamate and cysteine. In the second step, catalyzed by GSH-S, glycine is added to the C-terminal end of (gamma-EC). GSH is the predominant non-protein thiol in all nearly where it acts as an antioxidant in stress responses and as a mobile pool of reduced sulfur. It is also important in the regulation of plant growth and development.

Additional information: Total IgG concentration is 5,2 μg/ μl

Molecular Weight: 50 kDa

Gene ID: 828409

UniProt: Q8W4W3

Pathways: Cell RedoxHomeostasis