GCLC antibody

Catalog No. ABIN190704

This anti-GCLC antibody is a Rabbit Polyclonal antibody detecting GCLC in WB and IL. Suitable for Zea mays, Tomato, Nicotiana tabacum and Arabidopsis thaliana. This Primary Antibody has been cited in 2+ publications.

Quick Overview for GCLC antibody (ABIN190704)

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Reactivity: Zea mays, Tomato, Nicotiana tabacum, Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCLC antibody is un-conjugated

Application: Western Blotting (WB), Immunolocalization (IL)

Product Details anti-GCLC Antibody

Specificity: The antibody recognizes closely related tobacco class I isoforms: endochitinase ACHN-A (ca. 34 kDa) and endochitinase B CHN-B (ca. 32 kDa)

Cross-Reactivity (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Predicted Reactivity: dicots including:Pisum sativum, Riciuns communis, monocots including: Oryza sativa, Triticum aestivum

Characteristics: Expected / apparent Molecular Weight of the Antigene: 50 kDa
This antibody can be used as a marker of vacuolar contents.

Purification: affinity purified

Immunogen: KLH-conjugated synthetic peptide derived from Zea mays gamma-ECS Q8W4W3

Isotype: IgG

Application Details

Application Notes: 1: 5000 with standard ECL (WB), 1: 5000 (IL)

Comment:

antibodies has been used in immunolocalization on Arabidopsis thaliana

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water.

Buffer: PBS pH 7.4

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

References for anti-GCLC antibody (ABIN190704)

Sobrino-Plata, Carrasco-Gil, Abadía, Escobar, Álvarez-Fernández, Hernández: "The role of glutathione in mercury tolerance resembles its function under cadmium stress in Arabidopsis." in: Metallomics : integrated biometal science, Vol. 6, Issue 2, pp. 356-66, (2014) (PubMed).

Keefe, Hinz, Meins: "The effect of ethylene on the cell-type-specific and intracellular localization of β-1,3-glucanase and chitinase in tobacco leaves." in: Planta, Vol. 182, Issue 1, pp. 43-51, (2013) (PubMed).

Target Details for GCLC

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Alternative Name: Gamma-ECS

Background: AGI Code: At4g23100
Gamma-glutamylcysteine synthase (gamma-ECS) (EC 6.3.2.2) catalyses the first step of glutathione (GSH) synthesis, producing gamma-glutamylcysteine (gamma-EC) from L-glutamate and cysteine. In the second step, catalyzed by GSH-S, glycine is added to the C-terminal end of (gamma-EC). GSH is the predominant non-protein thiol in all nearly where it acts as an antioxidant in stress responses and as a mobile pool of reduced sulfur. It is also important in the regulation of plant growth and development.

Molecular Weight: 50 kDa

UniProt: Q8W4W3

Pathways: Cell RedoxHomeostasis