RPA1 antibody

Catalog No. ABIN190714

This Rabbit Polyclonal antibody specifically detects RPA1 in WB, IP and ChIP. It exhibits reactivity toward Saccharomyces cerevisiae and has been mentioned in 2+ publications.

Quick Overview for RPA1 antibody (ABIN190714)

Target: RPA1 (Replication Protein A1, 70kDa (RPA1))

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Product Details anti-RPA1 Antibody

Cross-Reactivity (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Predicted Reactivity: Saccharomyces cerevisiae

Characteristics: Expected / apparent Molecular Weight of the Antigene: 70 + 30 + 14 kDa

Purification: serum

Immunogen: RPA from Saccharomyces cerevisiae consisting of three subunits RFA1 (70 kDa), RFA2 (30 kDa) and RFA3 (14 kDa), overexpressed in E.coli and purified by chromatography, no affinity tags were added to any of three subunits

Application Details

Application Notes: 1: 20 000 with standard ECL (WB), chromatin immunoprecipiation

Comment:

antibody was also successfully used in ChIP application, presented data are courtesy of M. Pool and Dr. H. van Attikum

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

References for anti-RPA1 antibody (ABIN190714)

Chen, Lisby, Symington: "RPA coordinates DNA end resection and prevents formation of DNA hairpins." in: Molecular cell, Vol. 50, Issue 4, pp. 589-600, (2013) (PubMed).

Bentsen, Nielsen, Lisby, Nielsen, Gupta, Mundbjerg, Andersen, Bjergbaek: "MRX protects fork integrity at protein-DNA barriers, and its absence causes checkpoint activation dependent on chromatin context." in: Nucleic acids research, Vol. 41, Issue 5, pp. 3173-89, (2013) (PubMed).

Target Details for RPA1

Target: RPA1 (Replication Protein A1, 70kDa (RPA1))

Alternative Name: RFA

Background: Saccharomyces cerevisiaereplication protein A (RPA), also known as replication factor A (RFA) is a single-stranded DNA-binding protein that is required for multiple processes in eukaryotic DNA metabolism. Those processes include DNA replication, DNA repair, and recombination. Homologues to RPA have been identified in all eukaryotic organisms examined. RPA is heterotrimeric protein composed of subunits of approximately 70, 30, and 14 kDa. Members of this family bind nonspecifically to single-stranded DNA and interact with and/or modify the activities of multiple proteins. Alternative names: Replication protein A 69 kDa DNA-binding subunit, Single-stranded DNA-binding protein, DNA-binding protein BUF2, replication protein A 36 kDa subunit, DNA-binding protein BUF1 antibody

Molecular Weight: 70 + 30 + 14 kDa

UniProt: P22336, P26754

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA