PRTN3 antibody

Catalog No. ABIN190718

The Rabbit Polyclonal anti-PRTN3 antibody is suitable to detect PRTN3 in samples from Agrostis stolonifera, Nicotiana tabacum, Picea abies, Potato, Red pepper, Tomato and Vitis vinifera. It has been validated for WB, IL and Co-IP.

Quick Overview for PRTN3 antibody (ABIN190718)

Target: PRTN3 (Proteinase 3 (PRTN3))

Reactivity: Agrostis stolonifera, Nicotiana tabacum, Picea abies, Potato, Red pepper, Tomato, Vitis vinifera

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRTN3 antibody is un-conjugated

Application: Western Blotting (WB), Immunolocalization (IL), Co-Immunoprecipitation ( Co-IP)

Product Details anti-PRTN3 Antibody

Purpose: Anti-PR-3 / CHN | Class I chitinase

Specificity: Antibody is recognizing closely related tobacco class I isoforms: endochitinase ACHN-A (ca. 34 kDa) and endochitinase B CHN-B (ca. 32 kDa).

Cross-Reactivity (Details): Agostis stolonifera cv. 'Penncross', Capsicum annuum, Nicotiana tabacum, Picea abies, Solanum esculentum, Solanum lycopersicum, Solanum tuberosum, Vitis vinifera
Not reactive in: Cicer arietinum

Predicted Reactivity: Arabidopsis thaliana,Manihot esculenta,Zea mays

Purification: Protein G purified

Immunogen: Purified tobacco class I chitinase. The preparation used is a mixture of two class I isoforms (Shinshi et al.

Isotype: IgG

Application Details

Application Notes: 8 μg/mL (WB)

Comment:

Important note: For blocking 5 % skim milk in PBS without Ca++ should be used.This antibody is purified by affinity chromarography on Portein G.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water

Buffer: Total IgG. Protein G purified in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details for PRTN3

Target: PRTN3 (Proteinase 3 (PRTN3))

Alternative Name: PR-3

Background:

Background: Pathogenesis-related (PR) proteins, are induced in response to the infection of plants with microbial pathogens. Combinations of glucanase I and chitinase I are potent inhibitors of fungal growth in vitro however precise mechanism of that is still not known. Glucanase I (PR-2) and chitinase I (PR-3) contribute to defense against fungal infection and are currently used as markers for innate immunity, and in particular the ethylene/jasmonate signalling pathway in pathogenesis.

Additional information: Antibody is recognizing closely related tobacco class I isoforms: endochitinase A CHN-A (ca. 34 kDa) and endochitinase B CHN-B (ca. 32 kDa)This antibody can be used as a marker of vacuolar contents Keefe et al. (1990). The effect of ethylene on the cell-type-specific and intracellular localization of β-1,3-glucanase and chitinase in tobacco leave. Plant 182: 43-51.

Molecular Weight: 35, 34 | 32 and 34 kDa

Gene ID: 107759005

UniProt: P08252, P24091