PRTN3 antibody

Catalog No. ABIN190718

The Rabbit Polyclonal anti-PRTN3 antibody has been validated for WB. It is suitable to detect PRTN3 in samples from Nicotiana tabacum and Potato.

Quick Overview for PRTN3 antibody (ABIN190718)

Target: PRTN3 (Proteinase 3 (PRTN3))

Reactivity: Nicotiana tabacum, Potato

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRTN3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PRTN3 Antibody

Specificity: Antibody is recognizing closely related tobacco class I isoforms: endochitinase ACHN-A (ca. 34 kDa) and endochitinase B CHN-B (ca. 32 kDa).

Characteristics: Expected / apparent Molecular Weight of the Antigene: 35, 34 / 32 and 34 kDa

Purification: affinity purified

Immunogen: purified tobacco class I chitinase. The preparation used is a mixture of two class I isoforms (Shinshi et al., 1990, van Buuren et al., 1992): 1) Chitinase A (CHN A) P08252 encoded by gene chn48 derived from the N. tomentosiformis ancestor of tobacco. 2) Chitinase B (CHN B) P24091 encoded by gene chn50 derived from the N. sylvestris ancestor of tobacco.

Isotype: IgG

Application Details

Application Notes: Recommended Dilution: 8 ug/mL with standard ECL (WB).

Comment:

Important note: For blocking 5 % skim milk in PBS without Ca++ should be used.This antibody is purified by affinity chromarography on Portein G.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL of sterile water.

Buffer: PBS pH 7.4

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

Target Details for PRTN3

Target: PRTN3 (Proteinase 3 (PRTN3))

Alternative Name: PR-3

Background: Pathogenesis-related (PR) proteins, are induced in response to the infection of plants with microbial pathogens. Combinations of glucanase I and chitinase I are potent inhibitors of fungal growth in vitro however precise mechanism of that is still not known. Glucanase I (PR-2) and chitinase I (PR-3) contribute to defense against fungal infection and are currently used as markers for innate immunity, and in particular the ethylene/jasmonate signalling pathway in pathogenesis.

Molecular Weight: expected: 35 kDa, 34 kDa, apparent: 32 and 34 kDa

UniProt: P08252, P24091