GLU I (PR-2) antibody

Catalog No. ABIN190719

Cited in 1 publication. The Rabbit Polyclonal anti-GLU I (PR-2) antibody (ABIN190719) specifically detects GLU I (PR-2) in IL and WB. The antibody is reactive with Fragaria vesca, Lactuca sativa, Nicotiana benthamiana, Nicotiana clevelandii, Nicotiana glutinosa, Nicotiana tabacum, Phalaenopsis equestris, Poplar (Populus), Potato, Tomato and Vitis vinifera samples.

Quick Overview for GLU I (PR-2) antibody (ABIN190719)

Target: GLU I (PR-2)

Reactivity: Fragaria vesca, Lactuca sativa, Nicotiana benthamiana, Nicotiana clevelandii, Nicotiana glutinosa, Nicotiana tabacum, Phalaenopsis equestris, Poplar (Populus), Potato, Tomato, Vitis vinifera

Host: Rabbit

Clonality: Polyclonal

Application: Immunolocalization (IL), Western Blotting (WB)

Product Details

Purpose: Anti-PR-2 | GLU I | Class I beta-1,3-glucanase

Cross-Reactivity (Details): Fragaria vesca, Lactuca sativa, Nicotiana benthamiana, Nicotiana clevilandii, Nicotiana glutinosa, Nicotiana tabacum, Phalenopsis Sogo Yukidian cultivar V3, Populus sp., Solanum lycopersicum, Solanum tuberosum, Vitis vinifera
Not reactive in: Arabidopsis thaliana

Predicted Reactivity: Dicots,Oryza sativa,Prunus persica

Immunogen: Purified tobacco class I

Isotype: IgG

Application Details

Application Notes: 8 μg/mL (WB)

Comment:

Important note: for blocking 5 % skim milk in PBS without Ca++ should be used.This antibody is purified by affinity chromarography on Portein G.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water

Buffer: Total IgG in PBS pH 7.4. (without Ca++).

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References

Beffa, Neuhaus, Meins: "Physiological compensation in antisense transformants: specific induction of an \" glucan endo-1,3-beta-glucosidase in plants infected with necrotizing viruses." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 19, pp. 8792-6, (1993) (PubMed).

Target Details

Target: GLU I (PR-2)

Background:

Background: Pathogenesis-related (PR) proteins, are induced in response to the infection of plants with microbial pathogens. Combinations of glucanase I and chitinase I are potent inhibitors of fungal growth in vitro however precise mechanism of that is still not known. Glucanase I and chitinase I contribute to defense against fungal infection and are currently used as markers for innate immunity, and in particular the ethylene/jasmonate signalling pathway in pathogenesis. Alternative names of the protein: basic beta-1,3-glucanase

Additional information: For more details on immunolocalization, please referr to Keefe et al (1990). Plant 182: 43-51.This antibody can be used as a marker of vacuolar contents Keefe et al. (1990). The effect of ethylene on the cell-type-specific and intracellular localization of β-1,3-glucanase and chitinase in tobacco leave. Plant 182: 43-51.

Molecular Weight: 37 | 33 kDa

Gene ID: 107801151

UniProt: P27666