Superoxide dismutase copper chaperone antibody (cleaved)

Catalog No. ABIN190735

This Rabbit Polyclonal antibody specifically detects Superoxide dismutase copper chaperone in WB. It exhibits reactivity toward Arabidopsis thaliana, Mustard, Oryza sativa and Zea mays.

Quick Overview for Superoxide dismutase copper chaperone antibody (cleaved) (ABIN190735)

Target: Superoxide dismutase copper chaperone (CCS) (Copper Chaperone For Superoxide Dismutase (CCS))

Reactivity: Arabidopsis thaliana, Mustard, Oryza sativa, Zea mays

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Superoxide dismutase copper chaperone antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Superoxide dismutase copper chaperone Antibody

Binding Specificity: cleaved

Cross-Reactivity (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Predicted Reactivity: dicots including: Glycine max, Solanum tuberosum

Characteristics: Expected / apparent Molecular Weight of the Antigene: 33.8 / 37 kDa (Arabidopsis thaliana)

Purification: serum

Immunogen: The antibody was raised to the Arabidopsis thaliana full length CCs protein Q4ZJI5, fused to His-tag. Overexpressed using pET28a vector in Bl21 E. coli (Cd+) and purified using His-column. After purification, the His tag was cleaved using thrombin and then theprotein was purified using Resource-Q column connected to HPLC.

Application Details

Application Notes: 1 : 1000 with alkaline phosphatase (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water.

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Target Details for Superoxide dismutase copper chaperone

Target: Superoxide dismutase copper chaperone (CCS) (Copper Chaperone For Superoxide Dismutase (CCS))

Alternative Name: CCS

Background: AGI Code: At1g12520
Copper is an essential element for function of chloroplasts. It is a co-factor for superoxide dismutase (SOD) and for plastocyanin. Copper is transported to chloroplasts with a help of Copper Chaperones, which carry out the delivery of copper from transporters to targets. In the last step chaperone binds to a copper dependent enzyme and inserts the copper ions into its active site. The Arabidopsis thaliana gene AtCCS encodes a copper chaperone for SOD. The AtCCS protein was localized to chloroplasts where it may supply copper to the stromal Cu/ZnSOD.

Molecular Weight: expected: 33.8 kDa, apparent: 37 kDa (Arabidopsis thaliana)

UniProt: Q4ZJI5

Pathways: Transition Metal Ion Homeostasis